Prijenos G-virusa vinove loze i badnavirusa vinove loze 1 sjemenom vinove loze (Vitis vinifera L.)

master's thesis

• Title (english): Transmission of grapevine virus G and grapevine badnavirus 1 by grapevine seeds (Vitis vinifera L.)
• Author: Mateja Ćosić
• Mentor: Darko Vončina (mentor)
• Committee member: Darko Vončina (predsjednik povjerenstva)
• Committee member: Darko Preiner (član povjerenstva)
• Committee member: Edyta Đermić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant Pathology); Zagreb
• Defense date and country: 2022-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Vinova loza (Vitis vinifera L.), kao jedna od najuzgajanijih i gospodarski najznačajnih drvenastih kultura područja umjerene klime izložena je napadu velikog broja patogena među kojima se virusi izdvajaju kao jedni od važnijih. Jedan od glavnih načina širenja virusa vinove loze je razmjena zaraženog sadnog materijala. G-virus vinove loze (GVG) te badnavirus vinove loze 1 (GBV-1) utvrđeni su u relativno velikom postotku kod hrvatskih autohtonih sorata u našem priobalnom području. Kako bi se istražila mogućnost njihovog prijenosa sjemenom sa biljaka zaraženih navedenim virusima izdvojeno je sjeme koje je nakon obrade posijano u teglice sa supstratom. Dva mjeseca nakon klijanja biljke su testirane na prisutnost GVG odnosno GBV-1 metodom lančane reakcije polimerazom u realnom vremenu (qPCR). U slučaju GVG testirano je 100 biljaka, a u slučaju GBV-1 ukupno 420 biljaka. Provedenim laboratorijskim testiranjem nije utvrđena prisutnost niti jednog virusa čime nije potvrđena mogućnost njihovog prijenosa putem sjemena zaražene vinove loze.

Abstract (english)

Grapevine (Vitis vinifera L.), one of the most widely grown and economically important woody crops in temperate climates, is subject to attack by a variety of pathogens, among which viruses stand out as one of the most important. One of the main modes of transmission of grapevine viruses is the exchange of infected planting material. Grapevine virus G (GVG) and grapevine badnavirus 1 (GBV-1) were found in a relatively high percentage in Croatian autochthonous varieties grown in coastal area. To investigate the possibility of their transmission by seeds obtained from infected plants, seeds were collected and, after processing, sown in pots with a substrate. Two months after germination, the plants were tested for the presence of GVG and GBV-1 using real-time polymerase chain reaction (qPCR). In the case of GVG, 100 plants were tested and in the case of GBV-1, a total of 420 plants were tested. The laboratory tests did not confirm the presence of the virus, so the possibility of seed transmission in grapevine was not confirmed.

• Keywords: vinova loza; sjeme; prijenos; PCR u realnom vremenu
• Keywords (english): grapevine; seed; transmission; real-time PCR
• Language: croatian
• URN:NBN: urn:nbn:hr:204:458326
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-10-21 12:14:43