Ekonomski značajni virusi vinove loze u vinogradima u okolici Poreča

master's thesis

• Title (english): Economically important viruses in vineyards near Poreč
• Author: Kristian Đurić - Stjepanović
• Mentor: Darko Vončina (mentor)
• Committee member: Darko Vončina (predsjednik povjerenstva)
• Committee member: Edyta Đermić (član povjerenstva)
• Committee member: Darko Preiner (član povjerenstva)
• Committee member: Martin Jagunić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant Pathology); Zagreb
• Defense date and country: 2021-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

U svijetu je otkriveno preko 86 različitih vrsta virusa koji parazitiraju unutar vinove loze, od kojih samo mali broj predstavlja ekonomski značajne viruse. Predstavnici važnih virusnih bolesti vinove loze, ovisno o literaturi i regiji uzgoja, uglavnom se ubrajaju u osam virusa koji pripadaju trima skupinama: infektivna degeneracija (virus mozaika gušarke - ArMV, virus lepezastog lista vinove loze - GFLV), uvijenost lista (uvijenosti lista vinove loze pridruženi virusi 1,2 i 3 - GLRAV-1,2,3) i skupini naboranosti drveta vinove loze (A-virus vinove loze - GVA i B-virus vinove loze - GVB). Uz prethodno navedene pripadnike obično se pribraja i virus pjegavosti vinove loze (GFkV). Cilj istraživanja bio je utvrditi prisutnost ekonomski značajnih virusa vinove loze unutar vinograda u okolici Poreča. Uzorci su prikupljeni sa 7 različitih lokacija te su bili podvrgnuti testiranju korištenjem imuno-enzimske metode na čvrstoj fazi (ELISA). Potvrđena je prisutnost svih ekonomski značajnih virusa vinove loze. Najzastupljeniji među njima bio je GLRaV-3 (50,1%), zatim ga slijede: GVA (18,3%), GFkV (7,8%), GLRaV-1 (4,3%), GFLV (2,8%), GLRaV-2 (1,5%) te se na zadnjem mjestu nalazi prisutnost ArMV (0,3%). Također je utvđena prisutnost mješovitih infekcija gdje je najzastupljeniji kompleks GLRaV-3 + GVA - 16,6 %, s druge strane potvrđene su i druge kombinacije, ali njihov postotak nije bio veći od 6 %.

Abstract (english)

Over 86 different types of viruses that parasitize within the grapevine have been discovered worldwide, of which only a small number constitute economically significant viruses. Representatives of important grapevine viral diseases, depending on the literature and the region of cultivation, are mainly included in eight viruses that belong to three groups: infectious degeneration (Arabis mosaic virus - ArMV, Grapevine fanleaf virus - GFLV), grapevine leafroll (Grapevine leafroll-associated virus -1, 2 and 3 - GLRaV-1, 2 i 3) and rugose wood (A-grapevine virus - GVA and B-grapevine virus - GVB). In addition to the aforementioned groups, the Grapevine fleck virus (GFkV) is usually concluded. This study aimed to determine the presence of economically important grapevine viruses within vineyards in the vicinity of Poreč. Samples were collected from 7 different sites and were subjected to the enzyme-linked immunosorbent assay (ELISA). The presence of all economically important grapevine viruses was confirmed. The most common among them was GLRaV-3 (50,1%), followed by: GVA (18,3%), GFkV (7,8%), GLRaV-1 (4,3%), GFLV (2,8%), GLRaV-2 (1,5%) and in the last place is the presence of ArMV (0,3%) The presence of mixed infections was also confirmed, with GLRaV-3 + GVA - 16,6 % as most common combination. On the other hand, other mixed infections were confirmed, but their percantage did not exceed more than 6 %.

• Keywords: vinova loza; ekonomski značajni virusi; ELISA; mješovite infekcije
• Keywords (english): grapevine; economically important viruses; ELISA; mixed infections
• Language: croatian
• URN:NBN: urn:nbn:hr:204:872996
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-12-09 10:12:40