Utvrđivanje entomopatogenih nematoda na poljoprivrednim površinama kontinentalne Hrvatske

master's thesis

• Title (english): Determination of entomopathogenic nematodes on agricultural areas of continental Croatia
• Author: Viktorija Sever
• Mentor: Dinka Grubišić (mentor)
• Committee member: Dinka Grubišić (predsjednik povjerenstva)
• Committee member: Maja Čačija (član povjerenstva)
• Committee member: Ivan Juran (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Agricultural Zoology); Zagreb
• Defense date and country: 2021-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Entomopatogene nematode zemljišne su nematode koje se hrane bakterijama te zajedno mogu zaraziti i ubiti niz kukaca domaćina. Primjena entomopatogenih nematoda predstavlja manji rizik za neciljane organizme u odnosu na kemijske insekticide. Zbog toga su prihvatljive za okoliš te su idealne u biološkom suzbijanju štetnika. Najznačajnije entomopatogene nematode pripadaju porodicama Heterorhabditidae i Steinernematidae. Moguć je njihov uzgoj in vivo ili in vitro te se vrlo jednostavno mogu ispuštati u prirodu gdje se samostalno obnavljaju. Do sada su u Republici Hrvatskoj utvrđene vrste iz roda Steinernema u lokalitetima Arduševac, Karane i Đeletovci. Pretpostavka je da su prisutne i na drugim područjima u Republici Hrvatskoj, stoga je cilj ovoga rada utvrditi prisutnost entomopatogenih nematoda na poljoprivrednim površinama kontinentalne Hrvatske. Za potrebe ovoga rada prikupljeni su uzorci tla s devet lokaliteta kontinentalne Hrvatske. Postupak izolacije entomopatogenih nematoda proveden je prema metodici „Galleria bait“ i „White trap“. Ukupno je analizirano 45 uzoraka tla, a entomopatogene nematode iz roda Steinernema utvrđene su samo na lokalitetu Luka, u jednom uzorku, što iznosi 2,22 % od ukupno analiziranih uzoraka tla.

Abstract (english)

Entomopathogenic nematodes are soil nematodes that feed on bacteria. Together they can infect and kill several host insects. The use of entomopathogenic nematodes in plant protection poses a lower risk to non-target organisms compared to chemical insecticides. Therefore, they are environmentally friendly and are ideal for biological pest control. The most important entomopathogenic nematodes belong to the families Heterorhabditidae and Steinernematidae. Entomopathogenic nematodes can be produced through in vivo or in vitro processes and they can be very easily released into nature where they naturally renew themselves. So far, species of the genus Steinernema have been identified in the Republic of Croatia in the localities of Arduševac, Karane and Đeletovci. It is assumed that they are present in other areas in the Republic of Croatia, so the aim of this investigation was to determine the presence of entomopathogenic nematodes on agricultural land in continental Croatia. For the purposes of this work, soil samples were collected in nine localities of continental Croatia. The isolation procedure of entomopathogenic nematodes was carried out according to the methods of "Galleria bait" and "White trap". A total of 45 soil samples were analyzed, and entomopathogenic nematodes of the genus Steinernema were found only in one sample at the location Luka, which is 2.22 % of the total analyzed soil samples.

• Keywords: Steinernematidae; Heterorhabditidae; „Galleria bait“; voskov moljac; „White trap“
• Keywords (english): Steinernematidae; Heterorhabditidae; „Galleria bait“; greater wax moth; „White trap“
• Language: croatian
• URN:NBN: urn:nbn:hr:204:042455
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2021-12-07 08:17:46