Simptomatologija i karakterizacija izolata fitopatogene bakterije Pseudomonas syringae iz tkiva biljaka roda Prunus

master's thesis

• Title (english): Simptomatology and characterization of isolates of phytopathogenic bacteria pseudomonas syringae from plants of genus Prunus tissues
• Author: Helena Malović
• Mentor: Edyta Đermić (mentor)
• Committee member: Edyta Đermić (predsjednik povjerenstva)
• Committee member: Joško Kaliterna (član povjerenstva)
• Committee member: Martina Skendrović Babojelić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant Pathology); Zagreb
• Defense date and country: 2021-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Bakterija Pseudomonas syringae je uzročnik raka koštičavih voćnih vrsta koji se smatra jednom od najvažnijih bolesti koštičavih voćnih vrsta. Karakteristični, golim okom vidljivi simptomi zaraze su pojava rana na granama ili deblu uz obilno lučenje smole, a kod starijih stabala kora može biti ispucala, udubljena ili promijenjene boje. Problem predstavljaju latentne zaraze bakterijom P. syringae kada nema uočljivih simptoma, te se javlja potreba za izolacijom bakterije iz tkiva biljaka nakon čega slijedi njena identifikacija različitim testovima koji potvrđuju da se radi o bakteriji P. syringae i jasni su pokazatelj zaraze. U radu je dan prikaz simptoma bolesti raka koštičavih voćnih vrsta, te postupak i metode testiranja karakteristika izoliranih bakterijskih izolata iz prikupljenih uzoraka tkiva biljaka iz roda Prunus, s područja grada Zagreba. Posebno su istaknuti provedeni testovi hipersenzitivne reakcije na duhanu i biotest na jorgovanu. Rezultati testova su pokazali da je bakterija P. syringae uspješno izolirana u slučaju svih 8 klonskih linija bakterija čime je potvrđena hipoteza da izolati iz uzoraka s jače izraženim simptomima pokazuju izrazitu patogenost. Rad donosi i smjernice za nastavak determinacije bakterijskih izolata u smjeru određivanja zasebnih patovara i sojeva.

Abstract (english)

Bacterium Pseudomonas syringae is the causing agent of bacterial cancer of stone fruits, which is one of the most importante diseases of stone fruits. Characteristical symptoms of infection, visible by naked eye, are appearing in form of wounds on branches or tree trunk accompanied with huge production of gummosis, whether the bark of the older trees can be cracked, concaved or, of changed colour. The problem are latent infections with bacteria P. syringae when there are no visible symptoms, so there is a need for isolation of bacteria from plant tissues and tests that confirm bacterium P. syringae and are clear sign of the infection. In this work symptoms of bacterial cancer of stone fruit disease are described, as well as procedures and testing methods of characteristic properties of bacteria isolated from gathered tissue samples of plants of genus Prunus, from the Zagreb city area. Implementation of hypersensitive reaction on tobacco and biotest on lilac werw emphasized. Tests results showed that the bacteria P. syringae was successfully isolated in all of 8 clone bacterial lines which confirmed hypothesis that isolates from samples with stronger symptoms are showing stronger patogenicity. The thesis is giving directions for continuing of determination of bacterial isolates to separate pathovars and strains.

• Keywords: bakteriozni rak koštičavih voćnih vrsta; hipersenzitivna reakcija; biotest
• Keywords (english): bacterial cancer of stone fruits; hypersensitive reaction; biotest
• Language: croatian
• URN:NBN: urn:nbn:hr:204:775425
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2021-12-03 12:49:39