Osjetljivost repina buhača (Chaetocnema tibialis Illiger) na insekticide dozvoljene za primjenu u Republici Hrvatskoj

master's thesis

• Title (english): Sensitivity of flea beetle (Chaetocnema tibialis Illiger) to insecticides permitted for use in the Republic of Croatia
• Author: Paula Gluščić
• Mentor: Darija Lemić (mentor)
• Committee member: Darija Lemić (predsjednik povjerenstva)
• Committee member: Maja Čačija (član povjerenstva)
• Committee member: Renata Bažok (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Agricultural Zoology); Zagreb
• Defense date and country: 2020-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Repin buhač (Chaetocnema tibialis Illiger) radi velike štete u toplim i sušnim godinama na mladom usjevu šećerne repe, a izgrizanje rupica na lišću dovodi do propadanja biljaka i prorjeđivanja sklopa. Tretiranje sjemena insekticidima iz skupine neonikotinoida davalo je zadovoljavajuće rezultate, no zbog mogućeg negativnog učinka na pčele i pojave rezistentnosti, ti insekticidi su zabranjeni odlukom Europske komisije od 27. travnja 2018. za sve poljoprivredne usjeve na otvorenom. Stoga je bilo potrebno provjeriti osjetljivost repina buhača na insekticide koji imaju dozvolu za primjenu u RH: klorpirifos, lambda-cihalotrin i tiakloprid. Repin buhač prikupljen je na devet lokaliteta u istočnoj Hrvatskoj. Pokusi su provedeni po metodama IRAC 011 (piretroidi), IRAC 021 (neonikotinoidi), IRAC 025 (organo-fosforni insekticidi). Utvrđivana je učinkovitost preporučene doze, dvostruke doze i 1/5 preporučene doze insekticida u četiri repeticije. Postoje razlike u učinkovitosti između primijenjenih djelatnih tvari, najbolju učinkovitost ima klorpirifos. Na tiakloprid i lambda-cihalotrin repin buhač razvio je određeni stupanj rezistentnosti. Protokoli prema kojima se ocjenjuje stupanj rezistentnosti ne podrazumijevaju statističku analizu podataka te mogu dovesti do pogrešnog tumačenja rezultata. Populacije koje su kategorizirane kao rezistentne podvrgnute su statističkoj analizi te je utvrđeno da smanjena učinkovitost nije i statistički značajna. Metode je potrebno provjeravati, kao i provoditi redovita terenska istraživanja kojima se jedino pravovremeno može detektirati početak razvoja rezistentnosti te pravovremeno provesti antirezistentne strategije.

Abstract (english)

Flea beetle (Chaetocnema tibialis Illiger) causes big damage on young sugar beet crop in hot and dry years: biting holes in the leaves leads to plant decay and reduced plant population. Treatment of seeds with neonicotinoid insecticides gave satisfactory results, but due to the possible negative effect on bees and development of resistance, these insecticides were banned on 27 April 2018 by the European Commission for all outdoor agricultural crops. Therefore, it was necessary to check the sensitivity of sugar beet flea beetle to insecticides that are licensed for use in the Republic of Croatia: chlorpyrifos, lambda-cyhalothrin and thiacloprid. Flea beetles were collected at nine locations in eastern Croatia. The experiments were performed according to methods of IRAC 011 (pyrethroids), IRAC 021 (neonicotinoids) and IRAC 025 (organophosphorus insecticides). The effectiveness of the recommended dose, double dose and 1/5 of the recommended dose of insecticides was determined in four repetitions. There are differences in efficacy between the active substances used, chlorpyrifos has the best efficacy. The pest has developed a certain degree of resistance to thiacloprid and lambda-cyhalothrin. The protocols used to assess the degree of resistance do not involve statistical analysis of the data and may lead to misinterpretation of the results. Populations categorized as resistant were subjected to statistical analysis and it was found that the reduced efficiency was not statistically significant. The methods need to be checked, and field research should be conducted regularly, as this is the basic tool that can detect the beginning of the development of resistance in a timely manner and implement anti-resistance strategies in a timely manner.

• Keywords: klorpirifos; lambda-cihalotrin; repin buhač; rezistentnost; šećerna repa; tiakloprid
• Keywords (english): chlorpyrifos; lambda-cyhalothrin; flea beetle; resistance; sugar beet; thiacloprid
• Language: croatian
• URN:NBN: urn:nbn:hr:204:549613
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2020-10-09 07:26:06