Molekularna identifikacija gljiva izoliranih iz šipka (Punica granatum L.) sa simptomima citosporioznog sušenja

master's thesis

• Title (english): The molecular identification of fungi isolated from Pomegranate (Punica granatum L.) with symptoms cytosporic drying
• Author: Marijela Ivandić
• Mentor: Joško Kaliterna (mentor)
• Committee member: Joško Kaliterna (predsjednik povjerenstva)
• Committee member: Snježana Topolovec-Pintarić (član povjerenstva)
• Committee member: Jelena Gadže (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Plant Pathology); Zagreb
• Defense date and country: 2020-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Šipak je u Hrvatskoj biljna vrsta koja je relativno slabo istražena. Podložan je napadu mnogih patogena, od toga u najvećem broju fitopatogenih gljivama. Tijekom 2019. godine u Dalmaciji je uočeno sušenje šipaka što je prema simptomima, uočenima u prethodnim istraživanjima, upućivalo na citosporiozno sušenje uzrokovano fitopatogenom vrstom Cytospora punicae. Iako se vrsta Cytospora punicae smatra patogenom slabosti ona može uzrokovati i ozbiljna oštećenja i propadanja cijele biljke. Izolirane gljive identificirane su provizorno prema morfološkim obilježjima kolonija i konidija na PDA. Reprezentativni izolati su dodatno istraženi analizom DNA sekvenci molekularnog markera ITS primjenom metode PCR. Dobivene ITS sekvence su uspoređene s odgovarajućim sekvencama izolata C. punicae iz baze podataka GenBank primjenom bioinformatičkog programa BLAST, s za filogenetsku analizu je korištena metoda Maximum Likelihood. Svi analizirani izolati iz ovog istraživanja su identificirani kao vrsta C. punciae.

Abstract (english)

In Croatia, pomegranate is a plant species that is relatively poorly researched. It is susceptible to attack by many pathogens, of which in the largest number of phytopathogenic fungi. During 2019, drying of pomegranates was observed in Dalmatia, which according to the symptoms, observed in previous studies, indicated cytosporic drying caused by the phytopathogenic species Cytospora punicae. Although Cytospora punicae is considered a pathogen of weakness, it can also cause severe damage and decay of entire plants. Isolated fungi of identification are provisional according to the morphological characteristics of colonies and conidia on PDA. Representative isolates were further investigated by DNA sequence analysis of the ITS molecular market using PCR methods. The obtained ITS sequences were compared with the corresponding sequences of C. punicae isolates from the GenBank data base using the BLAST bioinformatics program and the Maximum Likelihood method was used for phylogenetic analysis. All analyzed isolates from this study were identified as C. punicae species.

• Keywords: šipak; Cytospora punicae; citosporiozno sušenje; gljive
• Keywords (english): pomegranate; Cytospora punicae; cytosporic drying; fungi
• Language: croatian
• URN:NBN: urn:nbn:hr:204:048571
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-10-07 09:30:22