Optimizacija i validizacija HPLC metode za određivanje laktoze u mlijeku

master's thesis

• Title (english): Optization and validation of HPLC method for determination of lactose in milk
• Author: Ivana Šarčević
• Mentor: Nataša Mikulec (mentor)
• Committee member: Nataša Mikulec (predsjednik povjerenstva)
• Committee member: Neven Antunac (član povjerenstva)
• Committee member: Aleksandra Perčin (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Dairy Science); Zagreb
• Defense date and country: 2020-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Cilj ovog rada bio je opisati mogućnosti kromatografskih analitičkih metoda u analitici mlijeka i mliječnih proizvoda te određivanje proširene mjerne nesigurnosti. Razvojem i validacijom metode za kvantitativno i kvalitativno određivanje laktoze u mlijeku HPLC-RID (tekućinska kromatografija s diferencijalnim refraktometrom ili detektorom indeksa loma) tehnikom, proveden je eksperimentalni dio rada. Prikazan je postupak validacije HPLC metode pomoću validacijskih parametara propisanih normom HRN ISO 22662:2010 koji su bili nužni kako bi se metoda ispravno valorizirala. Nakon utvrđenih parametara validacije za određivanje udjela laktoze u mlijeku analizirano je 30 uzoraka mlijeka. Na osnovu dobivenih rezultata po pojedinim parametrima validacije može se zaključiti da je HPLC metoda s RID detektorom brza, praktična i precizna metoda za određivanje sadržaja laktoze u mlijeku, te pouzdana za rutinsko korištenje u analitičkim laboratorijima. Rezultati ispitivanja uzoraka mlijeka su uspoređivani sa postojećom deklaracijom te isti upućuju da je metoda ispravno validirana i da daje valjane rezultate.

Abstract (english)

The aim of this paper was to describe the possibilities of chromatographic analytical methods in the analysis of milk and dairy products, and to determine the extended measurement uncertainty. By developing and validating a method for quantitative and qualitative determination of lactose in milk by HPLC-RID (Liquid Chromatography with Differential Refractometer or Refractive Index Detector) technique, the experimental part of the work was performed. The procedure of validation of the HPLC method using validation parameters prescribed by the standard HRN ISO 22662: 2010 is presented, which were necessary for the method to be properly valorized. A total of 30 milk samples were analyzed to determine the lactose content in milk after the validation procedure. Based on the obtained results by individual validation parameters, it can be concluded that the HPLC method with RID detector is a fast, practical and accurate method for determination of lactose content in milk, as well as reliable for routine use in analytical laboratories. The test results of the milk samples were compared with each existing label and indicated that the method is valid and delivers valid results.

• Keywords: kromatografija; validacija; HPLC; laktoza; mlijeko i mliječni proizvodi.
• Keywords (english): chromatography; validation; HPLC; lactose; milk and milk products.
• Language: croatian
• URN:NBN: urn:nbn:hr:204:695226
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-10-02 08:44:48