Rezistentnost krumpirove zlatice na insekticide različitih skupina u Koprivničko-križevačkoj i Međimurskoj županiji

master's thesis

• Title (english): Resistance of Colorado potato beetle to insecticides of different groups in Koprivnica-Križevci and Međimurje county
• Author: Ana Leš
• Mentor: Maja Čačija (mentor)
• Committee member: Maja Čačija (predsjednik povjerenstva)
• Committee member: Renata Bažok (član povjerenstva)
• Committee member: Darija Lemić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Agricultural Zoology); Zagreb
• Defense date and country: 2019-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Najznačajnijim štetnikom krumpira smatra se krumpirova zlatica koja je zbog intenzivnog
suzbijanja razvila rezistentnost na 56 djelatnih tvari insekticida. Cilj istraživanja bio je utvrditi
stanje rezistentnosti populacija zlatice sa šest lokacija iz Koprivničko-križevačke i
Međimurske županije. Laboratorijskim testovima utvrđena je učinkovitost četiri doze
pripravaka na osnovi djelatnih tvari klorpirifosa, cipermetrina, tiakloprida i spinosada nakon
48 sati te metaflumizona nakon 72 sata. Populacije su klasificirane u pet kategorija temeljem
učinkovitosti preporučene doze pripravka kao visoko osjetljive, osjetljive, blago rezistentne,
rezistentne i visoko rezistentne. Na klorpirifos je pet populacija bilo visoko rezistentno, a
jedna blago rezistentna. Cipermetrin je bio učinkovit samo na jednoj lokaciji u Međimurskoj
županiji, dok je na ostalima utvrđena rezistentnost. Tiakloprid je bio učinkovit na populacije s
tri lokacije, no u svakoj županiji utvrđene su i rezistentne populacije. Metaflumizon se
pokazao kao učinkovit u Koprivničko-križevačkoj županiji, gdje je samo jedna populacija bila
blago rezistentna, dok je u Međimurskoj županiji imao slabiji učinak, jer su dvije populacije
bile rezistentne do visoko rezistentne. Spinosad se pokazao kao najučinkovitiji insekticid za
suzbijanje krumpirove zlatice: sve istraživane populacije zlatice bile su osjetljive do visoko
osjetljive. Rezultati ukazuju da se stanje rezistentnosti na organofosforne insekticide i
piretroide nije promijenilo zadnjih 30 godina, ali i da neke populacije postaju rezistentne i na
novije insekticide, tiakloprid i metaflumizon. To pokazuje da zlatica relativno brzo postaje
rezistentna, što u budućnosti može dovesti do proširenja rezistentnosti na druge insekticide
istog ili sličnog mehanizma djelovanja, ali i na druga uzgojna područja krumpira, ukoliko se
isti insekticidi budu intenzivno primjenjivali.

Abstract (english)

The most important potato pest is Colorado potato beetle (CPB), which became resistant to
56 active substances due to intensive insecticide application. The aim of the study was to
determine the resistance status of CPB populations from six locations from Koprivnica-
Križevci and Međimurje counties. In laboratory tests, the efficacy of four different doses of
chlorpyrifos, cypermethrin, thiacloprid and spinosad was determined after 48 hours and
metaflumizone after 72 hours. Populations were classified into five categories based on the
efficacy of the recommended dose of the preparation as highly sensitive, sensitive, mildly
resistant, resistant and highly resistant. Five populations were highly resistant to chlorpyrifos
and one was mildly resistant. Cypermethrin was effective in only one location within
Međimurje County, while all other populations were resistant. Thiacloprid was effective on
populations from three locations, but resistant populations were also found in each county.
Metaflumizone proved to be very effective in the County of Koprivnica-Križevci, where only
one population was mildly resistant, while in Međimurje County it had a weaker effect
because two populations were resistant to highly resistant. Spinosad proved to be the most
effective insecticide for the CPB control: all the populations studied were sensitive to highly
sensitive. The results indicate that the resistance status to organophosphorus insecticides
and pyrethroids has not changed in the last 30 years, but also that some populations are
becoming more resistant to newer insecticides, thiacloprid and metaflumizone. This shows
that CPB becomes resistant relatively quickly, which in the future may lead to an extension
of resistance to other insecticides of the same or similar mechanism of action, as well as to
other potato growing areas if the same insecticides are intensively applied.

• Keywords: cipermetrin; klorpirifos; Leptinotarsa decemlineata; metaflumizon; rezistentnost; spinosad; tiakloprid
• Keywords (english): chlorpyrifos; cypermethrin; Leptinotarsa decemlineata; metaflumizone; resistance; spinosad; thiacloprid
• Language: croatian
• URN:NBN: urn:nbn:hr:204:643509
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-12-04 07:48:52