Određivanje S-alela kod lokalnih sorata trešnje pomoću DNA markera

master's thesis

• Title (english): Determination of S-alleles in local sweet cehrry varieties by DNA markers
• Author: Patricia Krpan
• Mentor: Aleš Vokurka (mentor)
• Committee member: Aleš Vokurka (predsjednik povjerenstva)
• Committee member: Martina Skendrović Babojelić (član povjerenstva)
• Committee member: Ivanka Habuš Jerčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Plant Breeding Genetics, Biometrics and Experimentation); Zagreb
• Defense date and country: 2019-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Iako suvremena proizvodnja trešanja daje prednost samooplodnim sortama koje se temelje na mutaciji S-alela, većina sorata ipak spada u stranooplodne sorte. To se posebno odnosi na tradicionalne lokalne sorte i na sve stare svjetski poznate sorte. Stoga, potpuni opis sorata uključuje i poznavanje sastava S-alela što je neophodan uvjet za zasnivanje proizvodnje i odabir adekvatnih sorata oprašivača. Analiza S-alela provedena je kod osam lokalnih genotipova trešnje s područja Banja Luke, uz tri slovenske sorte i nekoliko svjetski poznatih sorata koje su imale ulogu referentnih genotipova. Za detekciju S-alela pomoću molekularnih markera koristila se DNA izolirana iz mladog lisnog tkiva. Detekcija S-alela provedena je PCR amplifikacijom hipervarijabilnog segmenata S-lokusa. Fragmenti DNA su razdvojeni elektroforezom u agaroznom gelu i uspoređeni sa fragmentima referentnih genotipova, standardnom DNA za procjenu duljine DNA fragmenata umnoženih PCR-om, i s literaturnim izvorima. Utvrđen je sastav S-alela kod istraženih genotipova, a za jedan genotip (Ašlamka 2) je ustanovljena jedinstvena kombinacija S-alela S10S13 koja nije zabilježena niti u jednom literaturnom navodu u svijetu. Riječ je o stranooplodnim genotipovima pa je na temelju utvrđenog sastava S-alela dana preporuka za moguće sorte oprašivače među onim sortama koje su dostupne.

Abstract (english)

Although modern cherry sweet cherry production favours self-compatible varieties based on S-allele mutations, the majority of sweet cherry varieties still belong to the group of self-incompatible varieties. This is especially true for traditional local varieties and all of the old world-known varieties. Therefore, the full description of varieties includes knowledge about the composition of S-alleles, which is a prerequisite for setting up the production and the choice of suitable pollinators. The analysis of S-alleles was carried out for eight genotypes from the region of Banja Luka, alongside with three Slovenian varieties and several world known varieties which were included as referral genotypes. The DNA extracted from young leaf tissue was used for the detection of S-alleles by molecular markers. The detection of S-alleles was carried on by PCR amplification of hypervariable segments of S-loci. The fragments of DNA were separated by electrophoresis in the agarose gel and compared with the fragments of referral genotypes, with the standard DNA for the estimation of the length of the PCR amplified fragments, and with literature data. The composition of S-alleles was determined in all genotypes included into the research. A unique combination of S-alleles, S10S13, not known in the literature until now, was determined for one genotype (Ašlamka 2) from Bosnia. For all the genotypes, which are self-incompatible, a recommendation for the most suitable varieties-pollinators was made among the available varieties

• Keywords: trešnja; Prunus avium L.; S-aleli; DNA markeri; PCR
• Keywords (english): sweet cherry; Prunus avium L.; S-allele; DNA markers; PCR
• Language: croatian
• URN:NBN: urn:nbn:hr:204:137457
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-11-20 07:27:53