Kemijska karakterizacija pčelinjeg otrova primjenom infracrvene (FTIR-ATR) spektroskopije

master's thesis

• Title (english): Chemical characterization of honey bee venom using infrared (FTIR-ATR) spectroscopy
• Author: Marko Levanić
• Mentor: Lidija Svečnjak (mentor)
• Committee member: Lidija Svečnjak (predsjednik povjerenstva)
• Committee member: Dragan Bubalo (član povjerenstva)
• Committee member: Marko Vinceković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Fisheries, Beekeeping, Game Management and Special Zoology); Zagreb
• Defense date and country: 2019-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Pčelinji je otrov (apitoksin) vrijedan proizvod medonosne pčele (Apis melifera L.) sa širokom primjenom i rastućom potraţnjom u farmaceutskoj i kozmetičkoj industriji, Glavninu njegova sastava čine peptidi i enzimi (60-70%). Cilj je ovog rada bio provesti detaljnu kemijsku karakterizaciju otrova medonosne pčele metodom infracrvene spektroskopije. Uzorci pčelinjeg otrova prikupljeni su iz 3 pokusne pčelinje zajednice izravno iz ţalca pčela radilica različite dobi i metodom elektrostimulacije pčela (skupni uzorak) te analiziran metodom FTIR-ATR spektroskopije. Rezultati su pokazali kako pčelinji otrov daje karakterističan FTIR-ATR spektralni profil u kojem dominiraju apsorpcijske vrpce proteina (enzima) i peptida. Rezultati spektralne analize pčelinjeg otrova pčela radilica različite dobi (7, 14, 24 i 42 dana) pokazali su kako je kvalitativni i kvantitativni sastav otrova postojan tijekom cijelog ţivota pčele, dok je analiza skupnog uzorka otrova prikupljenog iz pokusnih zajednica ukazala na kontaminaciju prikupljenog otrova pčelinjim voskom i propolisom.

Abstract (english)

Bee venom (apitoxin) is a valuable honey bee (Apis melifera L.) product characterized by wide use and growing demand in pharmaceutical and cosmetic industry. It is predominantly consisted of peptides and enzymes (60-70%). The aim of this study was to perform a detailed chemical characterization of honey bee venom using infrared spectroscopy. Samples of bee venom were collected from 3 experimental honey bee colonies directly from the sting of worker bees of different age, and by bee electrostimulation method (pooled sample), and analysed by FTIR-ATR spectroscopy. The results have revealed that bee venom provides a characteristic FTIR-ATR spectral profile dominated by absorption bands of proteins (enzymes) and peptides. The results of the spectral analysis of bee venom of workers of different age (7, 14, 24 and 42 days old) showed that the qualitative and quantitative composition of the bee venom is stable throughout the life of the honey bee, while the analysis of a pooled venom sample collected from the experimental colonies indicated contamination of the collected venom with beeswax and propolis.

• Keywords: medonosna pčela; pčelinji otrov; FTIR-ATR spektroskopija; kemijska karakterizacija
• Keywords (english): honey bee; bee venom; FTIR-ATR spectroscopy; chemical characterization
• Language: croatian
• URN:NBN: urn:nbn:hr:204:778601
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-11-08 07:15:30