Utjecaj različitih vrsta svjetla na kvalitetu mikropropagiranih kapara (Capparis orientalis Veill.)

master's thesis

• Title (english): Effect of different types of light on the quality of micropropagated capers (Capparis orientalis Veill.)
• Author: Kristina Bekavac
• Mentor: Anita Bošnjak Mihovilović (mentor)
• Committee member: Anita Bošnjak Mihovilović (predsjednik povjerenstva)
• Committee member: Snježana Kereša (član povjerenstva)
• Committee member: Tatjana Prebeg (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Plant Breeding Genetics, Biometrics and Experimentation); Zagreb
• Defense date and country: 2019-05-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Kapar (Capparis orientalis Veill.) je grmolika trajnica koja raste uglavnom u mediteranskim zemljama. Ima važnu ulogu u prehrambenoj industriji i postao je skupi i komercijalno vrije-dan proizvod. Konvencionalna metoda razmnožavanja kapara putem sjemena nije poželjna uglavnom iz dva razloga: niskog postotka klijavosti zbog dormantnosti sjemena i stranooplo-dnje koja za posljedicu ima visoki stupanj heterozigotnosti sjemena. Za plantažni uzgoj pot-rebno je osigurati veliki broj sadnica za što se kao rješenje nameće mikropropagacija. Cilj ovog istraživanja bio je istražiti utjecaj tri različita izvora svjetla na zakorjenjivanje kapara in vitro te na uspješnost aklimatizacije mikropropagiranih kapara u ex vitro uvjetima. U poku-sima su kao eksplantati korišteni uniformni izdanci mikropropagiranih kapara duljine 1-1,5 cm uzgajani na MS mediju s dodatkom 0,6 mg/l meta-topolina (mT). Korištena su tri izvora umjetnog osvjetljenja: fluorescentne cijevi; LED rasvjeta s omjerom crvenog i plavog spektra 70% : 30%; LED svjetlo širokog spektra: 35 % crvenog: 21% plavog: 39% zelenog: 6% daleko crvenog spektar. Rezultati provedenog istraživanja upućuju da je LED osvjetljenje širokog spektra pozitivno utjecalo na mjerene karakteristike korijena: dužinu korijena, površinu i volumen korijena kao i na gustoću puči i koncentraciju fotosintetskih pigmenata. Utvrđeno je da nema značajne razlike u postotku aklimatiziranih biljaka među tretmanima.

Abstract (english)

Caper (Capparis orientalis Veill.) is perennial shrub that grows predominantly in the Mediterranean region. Capers play an important role in food production and have become highly valuable commercial product. Conventional method of caper reproduction by planting seeds is not desirable for two main reasons: low germination rate due to the dormancy of the seed and cross-pollination, which results in a high level of heterozygous seed. Cultivation of capers requires large quantity of seedlings and these quantities can be supplied by micropropagation. The aim of this study was to explore the influence of three sources of lights on rooting of capers in vitro and on success of acclimatization of micropropagated capers in ex vitro conditions. During experiments, uniformed sprouts of micropropagated capers of 1-1,5 cm in length have been used as explants and have been cultivated on an MS medium with addition of 0,6 mg/l meta-Topolin (mT). Three sources of artificial light have been used in experiments: fluorescent lamps; LED lights with red to blue ratio of 70% to 30%; LED broad spectrum light: 35 % of red: 21% of blue: 39% of green: 6% of far red light. The results of research indicate that LED broad spectrum light has a positive impact on the measured root characteristics: root length, surface and volume of roots as well as on density of stomata and concentration of photosynthetic pigments. The results also indicate that treatments do not result in significant differences in percentage of acclimatized plants.

• Keywords: kapar; Capparis orientalis Veill.; mikropropagacija; fluorescentne cijevi; LED rasvjeta
• Keywords (english): Caper; Capparis orientalis Veill.; micropropagation; fluorescent tubes; LED lights
• Language: croatian
• URN:NBN: urn:nbn:hr:204:017159
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-07-15 07:25:34