Akumulacija žive u samoniklim jestivim gljivama na području Istarske i Primorsko-goranske županije

master's thesis

• Title (english): Accumulation of mercury in wild edible mushrooms in the area of Istria and Primorsko-goranska county
• Author: Luka Rakić
• Mentor: Ivan Širić (mentor)
• Committee member: Ivan Širić (predsjednik povjerenstva)
• Committee member: Milan Poljak (član povjerenstva)
• Committee member: Božidar Benko (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Animal Science and Technology); Zagreb
• Defense date and country: 2018-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Cilj ovog istraživanja bio je utvrditi koncentracije žive u 10 samoniklih vrsta jestivih gljiva i tlu na kojem rastu i prikladnost analiziranih vrsta gljiva kao bioindikatora onečišćenja okoliša. U istraživanju je analizirano pet saprofitskih vrsta (Agaricus campestris (L) Fries, Armilariella mellea (Vahl. ex Fr.) Karst., Clitocybe inversa (Scop. ex Fr.) Pat., Clitocybe nebularis Batsch. ex Fr., Macrolepiota procera (Scop. ex Fr.) Sing.,) i pet ektomikoriznih vrsta (Lactarius deterimus Groger, Boletus edulis Bull. ex Fries, Boletus aestivalis Paulet ex Fries, Tricholoma portentosum (Fr.) Quelet, Tricholoma terreum (Schff. ex Fr.) Kummer). Koncentracija žive u otopini koja je prilagođena za očitavanje pojedinog elementa, utvrđena je mjerenjem apsorpcije na atomskom apsorpcijskom spektrometru AAS (SOLAR-THERMO SCIENTIFIC, Type M5 AA System) sukladno normi HRN ISO 11466: 2004.
Utvrđena je značajna razlika (p<0,05) u ukupnoj koncentraciji žive između saprofitskih i ekotomikoriznih vrsta gljiva. Najviša prosječna koncentracije žive utvrđena je u vrsti Boletus aestivalis na lokalitetu Otok Krk (2,01 mg kg-1) i Labinština (2,02 mg kg-1).Ustanovljene su značajne razlike u prosječnoj distribuciji žive između anatomskih dijelova plodnog tijela. Značajno veći sadržaj žive utvrđen je u klobuku u odnosu na stručak (p<0,001). Bioakumulacijska svojstva ustvrđena su za sve analizirane vrste gljiva (BCF>1), pri čemu su najbolji bioindikatori onečišćenja okoliša živom gljive iz roda Boletus. Usporedbom koncentracija žive u gljivama i propisanim zakonskim odredbama, može se zaključiti da konzumacija istih ne predstavlja negativan učinak na zdravlje ljudi.

Abstract (english)

The aim of this study was to determine the concentration of mrcury in ten wild edible mushrooms and the substrate, to assess the role of individual species as biological indicators of environmental pollution. In this study we analyzed five saprophytic species (Agaricus campestris (L) Fries, Armilariella mellea (Vahl. ex Fr.) Karst., Clitocybe inversa (Scop. ex Fr.) Pat., Clitocybe nebularis Batsch. ex Fr., Macrolepiota procera (Scop. ex Fr.) Sing.,) and five ectomycorrhizal species (Boletus aestivalis Paulet ex Fries., Boletus edulis Bull ex Fries., Lactarius deterrimus Groger., Tricholoma portentosum (Fr.) Quelet, Tricholoma terreum (Schff. ex Fr.) Kummer). The concentration of mercury in a solution adapted to read the individual element was determined by measuring absorption at the atomic absorption spectrometer AAS (SOLAR-THERMO SCIENTIFIC, type M5 AA System) according to HRN ISO 11466: 2004. Significant differences were found in the concentrations of mercury between analysed saprophytic and ectomycorrhizal species of mushrooms (p<0,05). The highest mean concentrations of mercury was determined in Boletus aestivalis species in area Island Krk (2,01 mg kg-1) and Labinstina (2,02 mg kg-1). The average distribution rates of mercury inanatomical parts of the fruiting body were different. Significantly higher concentration of mercury was determined in the cap in relation to stipe of analyzed mushrooms (p<0,001). Bio-accumulation features were established in all of the investigated mushroom species (BCF>1), whereby the best bio-indicators of environmental pollution of mercury mushrooms from the genus Boletus. By comparing the concentration of mercury in mushrooms and the legal act, it can be concluded that consuming them does not have a negative impact on human health.

• Keywords: AAS; jestive gljive; toksikološki rizik; živa
• Keywords (english): AAS; edible mushrooms; toxicological risk; mercury
• Language: croatian
• URN:NBN: urn:nbn:hr:204:384858
• Study programme: Title: Organic Agriculture with Agrotourism; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka ekološke poljoprivrede i agroturizma (magistar / magistra inženjer/inženjerka ekološke poljoprivrede i agroturizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-10-17 12:51:28