Određivanje podrijetla primki graha trešnjevca pomoću fazeolina

master's thesis

• Author: Katarina Krenos
• Mentor: Klaudija Carović-Stanko (mentor)
• Committee member: Klaudija Carović-Stanko (predsjednik povjerenstva)
• Committee member: Zlatko Šatović (član povjerenstva)
• Committee member: Jerko Gunjača (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Plant biodiversity); Zagreb
• Defense date and country: 2017-02-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Premda u Hrvatskoj postoje povoljni agroekološki uvjeti za uzgoj graha (Phaseolus vulgaris L.), činjenica je da je u našoj zemlji ova kultura zapostavljena i da su površine pod grahom nedostatne. Glavni skladišni protein graha, fazeolin, sastoji se od kompleksa heterogenih glikoproteina s različitim sastavom polipeptida, koje kodira porodica gena. Ovo istraživanje se temeljilo na izolaciji fazeolina pomoću specifičnih PCR početnica s kojima je moguće umnožiti sekvencu gena fazeolina, analizirati razlike u obrascu umnoženih ulomaka i odrediti tip fazeolina. U istraživanje je bilo uključeno 107 primki tradicijskog kultivara graha 'Trešnjevac' iz različitih županija Hrvatske kojima je određen tip fazeolina. Utvrđeno je da sve primke tradicijskog kultivara graha 'Trešnjevac' pripadaju andskom podrijetlu. U gotovo svim županijama većina primki (72,90 %) pripada fazeolin tipu III ("T"), dok 27,10 % primki pripada fazeolin tipu II ("H" ili "C"). Iznimka je Krapinsko-zagorska županija, čije primke, većinom pripadaju fazeolin tipu II ("H" ili "C"). Također je utvrđeno da visoki habitus rasta imaju primke koje pripadaju fazeolin tipu II ("H" ili "C"), a niski habitus rasta imaju primke koje pripadaju fazeolin tipu III ("T").

Abstract (english)

Although in Croatia there are favourable environmental conditions for common bean (Phaseolus vulgaris L.) cultivation, the fact is that this culture is neglected in our country and the area under beans is insufficient. Phaseolin is the major storage protein of the common bean and it consists of complex heterogeneous glycoproteins with different composition of a polypeptide encoded by a gene family. Research was carried out on isolation of phaseolin using specific PCR primers with which is possible to multiply the sequence of genes phaseolin, analyse the differences in the pattern of amplified fragments and determine the type of phaseolin. Research included 107 accessions of Croatian common bean landrace 'Trešnjevac' originated from different counties of Croatia. All of the accessions of common bean landrace 'Trešnjevac' have the Andean origin. Most of the accessions (72,90 %), from all of the Counties, had phaseolin type III ("T"), while only one third of the accessions had phaseolin type II ("H" or "C"). The exception is Krapinsko-zagorska County in which the most of accessions had phaseolin type II (H or C). Accessions with climbing growth habit had phaseolin type II ("H" or "C") while accessions with determinate bush habit had phaseolin type III ("T").

• Keywords: fazeolin; grah.; Phaseolus vulgaris L.; podrijetlo; tradicijski kultivar
• Keywords (english): common bean; landraces; origin; phaseolin; Phaseolus vulgaris L.
• Language: croatian
• URN:NBN: urn:nbn:hr:204:131247
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-02-24 10:47:52