Obabir biljega za analizu testova očinstva u konzervacijskom uzgoju riječne kornjače

master's thesis

• Title (english): Selecting the markers for the paternity tests in conservation breeding of the balkan terrapin
• Author: Dora Krstulović
• Mentor: Dragica Šalamon (mentor)
• Committee member: Dragica Šalamon (predsjednik povjerenstva)
• Committee member: Alen Džidić (član povjerenstva)
• Committee member: Filip Varga (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Animal Science I); Zagreb
• Defense date and country: 2022-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

M. rivulata je ugrožena vrsta zaštićena Direktivom o staništima. Cilj ovog rada je pronalazak genetičkih alata za upravljanje genetskom raznolikosti ove vrste. Istraženi su literatura i otvoreni genetski repozitoriji te su analizirani genotipovi mikrosatelitnih biljega kornjača iz hrvatskih populacija u svrhu ocjene mogućnosti njihove uporabe za potrebe testova očinstva. Za vrstu je raspoloživo znanje o sekvencama mitohondrijskih gena, cjelovitih i necjelovitih te anonimnih gena, mikrosatelitnih biljega, rRNK i cjelovite mtDNA. Pregled 18 mikrosatelitnih biljega pokazuje da su dovoljno polimorfni i raspoloživi u dovoljnom broju. Analiza genotipova iz Hrvatske pokazuje veliku vjerojatnost pojave nul-alela kod 9 od 15 uspješno umnoženih biljega. Populacijski parametri za konavosku, majkovsku i sutorinsku populaciju izračunati su na 6 mikrosatelitnih biljega. SNP biljezi razvijeni ddRAD tehnikom omogućili bi izračun efektivne veličine populacije i efikasno upravljanje populacijama.

Abstract (english)

M. rivulata is an endangered species protected by the Habitats Directive. The aim of this work is to find genetic tools for managing its genetic diversity. Literature and open genetic repositories were investigated, and the microsatellite markers genotypes from Croatian populations were analysed to evaluate the possibility of their use for the purposes of paternity tests. For the species, available sequences include mitochondrial genes, complete and incomplete genes, microsatellite markers, rRNA and complete mtDNA. A review of the 18 microsatellite markers shows that they are sufficient in number and polymorphic. Genotypes from Croatia show a high probability of occurrence of null alleles in 9 out of 15 successfully amplified markers. Population parameters for the Konavle, Majkovi and Sutorina populations were calculated using 6 microsatellite markers. SNP markers developed using the ddRAD would enable the estimation of the effective population size and efficient population management.

• Keywords: molekularni biljezi; mikrosateliti; upravljanje populacijom; zaštita prirode
• Keywords (english): molecular markers; microsatellites; population management; nature protection
• Language: croatian
• URN:NBN: urn:nbn:hr:204:430415
• Study programme: Title: Animal Genetics and Breeding; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka genetike i oplemenjivanja životinja (magistar/ magistra inženjer/inženjerka genetike i oplemenjivanja životinja)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2022-11-25 09:31:42