Utjecaj toplinske obrade na sastav i kakvoću pčelinjeg voska

master's thesis

• Title (english): Influence of heat treatment on beeswax composition and quality
• Author: Gabrijela Findrik
• Mentor: Lidija Svečnjak (mentor)
• Committee member: Lidija Svečnjak (predsjednik povjerenstva)
• Committee member: Dragan Bubalo (član povjerenstva)
• Committee member: Marko Vinceković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Fisheries, Beekeeping, Game Management and Special Zoology); Zagreb
• Defense date and country: 2022-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Tehnologija proizvodnje satnih osnova uključuje sterilizaciju voska, odnosno izlaganje visokim temperaturama (najčešde 120-140 °C) kako bi se uništile spore bakterije Paenibacillus larvae, uzročnika američke gnjilode pčelinjeg legla. Spomenuta toplinska obrada u određenoj mjeri može utjecati na kakvodu voska, stoga je cilj ovog rada bio istražiti utjecaj različitih toplinskih tretmana na sastav i kakvodu pčelinjeg voska. Istraživanje je bilo provedeno na uzorcima sirovog pčelinjeg voska (n=5) koji su bili izloženi različitim toplinskim tretmanima s obzirom na temperaturu (100 °C, 120 °C, 140 °C i 160 °C) i duljinu trajanja toplinske obrade (30 minuta, 1 sat, 3 sata). Ukupni kemijski sastav uzoraka analiziran je infracrvenom spektroskopijom (FTIR-ATR tehnikom snimanja spektara). Rezultati spektralne analize pokazali su kako vedina primijenjenih toplinskih tretmana nije imala značajan utjecaj na cjelokupan kemijski sastav i kakvodu pčelinjeg voska. Međutim, utvrđen je negativan utjecaj izlaganja pčelinjeg voska temperaturi od 160 °C u trajanju od 3 sata na udio slobodnih masnih kiselina u pčelinjem vosku (degradacija frakcije slobodnih masnih kiselina). Također, s porastom temperature zapažen je i porast udjela estera.

Abstract (english)

The technology of producing beeswax foundation includes beeswax sterilization which represents exposure to high temperatures (mostly between 120-140 °C) in order to destroy the spores of Paenibacillus larvae, bacteria causing American foulbrood. Mentioned heat treatment can affect beeswax composition and quality to some extent, so the aim of this study was to examine the influence of different heat treatments on beeswax composition and quality. The study was carried out on beeswax samples (n=5) which were exposed to different heat treatments with regard to the temperature (100 °C, 120 °C, 140 °C and 160 °C), and treatment duration (30 minutes, 1 hour and 3 hours). The overall chemical composition of beeswax samples was analyzed by infrared spectroscopy (FTIR-ATR recording technique). The results of the spectral analysis showed that most of heat treatments did not have a significant impact on the overall chemical composition and quality of beeswax. However, a negative influence of beeswax exposure to a temperature of 160 °C for 3 hours on the proportion of free fatty acids (degradation of the fraction of free fatty acids) in beeswax was observed. Furthermore, with the increase in temperature, an increase in the proportion of esters was also observed.

• Keywords: pčelinji vosak; toplinska obrada; kemijski sastav; kakvoda; FTIR-ATR spektroskopija
• Keywords (english): beeswax; heat treatment; chemical composition; quality; FTIR-ATR spectroscopy
• Language: croatian
• URN:NBN: urn:nbn:hr:204:581814
• Study programme: Title: Fisheries and Game Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka ribarstva i lovstva (magistar/ magistra inženjer/inženjerka ribarstva i lovstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-10-18
• Created on: 2022-10-18 09:22:49