Djelovanje apitoksina na višestruko otporne bakterije

master's thesis

• Title (english): The effect of apitoxins on multidrug-resistant bacteria
• Author: Nikolina Čukac
• Mentor: Mirna Mrkonjić Fuka (mentor)
• Committee member: Mirna Mrkonjić Fuka (predsjednik povjerenstva)
• Committee member: Lidija Svečnjak (član povjerenstva)
• Committee member: Dragan Bubalo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Microbiology); Zagreb
• Defense date and country: 2022-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Nekritička uporaba antibiotika uzrokovala je globalnu pojavu rezistencije bakterija na konvencionalne antibiotike. Stoga je potrebno pronaći alternativna rješenja za sprječavanje njihovog rasta. U ovom je radu ispitan inhibitorni utjecaj uzoraka apitoksina (n=3) na sojeve višestruko rezistentnih bakterija Acinetobacter baumanii, Klebsiella pneumoniae, Enterococcus faecium i Staphylococcus aureus (n=20). Zone inhibicije rasta, određene metodom difuzije agara u bunaru, pokazuju da su Gram pozitivne bakterije osjetljivije (11,9 mm ± 0,7 mm) na djelovanje apitoksina od Gram negativnih bakterija (10,8 mm ± 4,1 mm; ANOVA, Bonferroni test: p = 0,03). Minimalna inhibitorna (MIK) i minimalna baktericidna (MBK) koncentracija uzoraka apitoksina iznosile su između 0,13 i 31,25 μg/ml. Utvrđeno je kako su uzorci apitoksina djelotvorni u inhibiciji rasta svih ispitivanih vrsta, ali pri različitim koncentracijama. Rezultati su pokazali i kako nema značajne razlike u inhibiciji različitih sojeva iste vrste, kao ni između djelotvornosti različitih uzoraka apitoksina.

Abstract (english)

The uncritical use of antibiotics has caused the global emergence of bacterial resistance to conventional antibiotics. Therefore, it is necessary to find alternative solutions to prevent their growth. In this work, the inhibitory effect of apitoxin samples (n=3) on strains of multi-resistant bacteria Acinetobacter baumanii, Klebsiella pneumoniae, Enterococcus faecium and Staphylococcus aureus (n=20) was tested. Zones of growth inhibition, determined by the agar diffusion method in the well, show that Gram-positive bacteria are more sensitive (11.9 mm ± 0.7 mm) to the action of apitoxin than Gram-negative bacteria (10.8 mm ± 4.1 mm; ANOVA, Bonferroni test: p = 0.03). The minimum inhibitory (MIC) and minimum bactericidal (MBK) concentrations of apitoxin samples were between 0.13 and 31.25 μg/ml. It was found that apitoxin samples are effective in inhibiting the growth of all tested species, but at different concentrations. The results showed that there is no significant difference in inhibition between different strains of the same species, as well as between the effectiveness of different samples of apitoxin.

• Keywords: antibiotički rezistentne bakterije; apitoksin; minimalna inhibitorna koncentracija (MIK); minimalna baktericidna koncentracija (MBK)
• Keywords (english): antibiotic-resistant bacteria; apitoxin; minimum inhibitory concentration (MIC); minimum bactericidal concentration (MBC)
• Language: croatian
• URN:NBN: urn:nbn:hr:204:990818
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-10-13
• Created on: 2022-10-13 10:36:01