Utjecaj inokulacije autohtonim sojevima Rhizobium leguminosarum na nodulaciju i prinos graha

master's thesis

• Title (english): The effect of inoculation with indigenous Rhizobium leguminosarum strains on nodulation and yield of common bean
• Author: Tea Pavić
• Mentor: Sanja Sikora (mentor)
• Committee member: Sanja Sikora (predsjednik povjerenstva)
• Committee member: Nina Toth (član povjerenstva)
• Committee member: Mihaela Blažinkov (član povjerenstva)
• Committee member: Ivana Rajnović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Microbiology); Zagreb
• Defense date and country: 2021-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Premda je grah vrlo značajna, visokovrijedna povrtna kultura, i u Hrvatskoj postoje povoljni agroekološki uvjeti za njegov uzgoj, neopravdano je zapostavljen te na tržištu dominira uvezeni grah. Korištenje procesa simbiozne fiksacije dušika može značajno doprinijeti ekološki prihvatljivoj i ekonomski opravdanoj proizvodnji graha. Cilj ovog rada je utvrditi simbiozna svojstva različitih sojeva Rhizobium leguminosarum u agroekološkom području Koprivničko-križevačke županije. Istraživanje je provedeno putem poljskog pokusa, a faktori pokusa bili su sojevi R. leguminosarum (četiri autohtona soja, referentni soj i kontrola) i sorte graha ('Alida' i 'Marina'). Tijekom pokusa određivana je nodulacijska sposobnost sojeva, masa nadzemnog dijela biljke, broj mahuna po biljci, broj zrna u mahuni te prinos zrna graha. Utvrđen je statistički opravdan utjecaj sorte na sva istraživana svojstva graha. Sorta 'Alida' signifikantno je bolja u odnosu na sortu 'Marina' po svim svojstvima osim u broju zrna u mahuni. Dobiveni rezultati su pokazali da su svi istraživani sojevi kompatibilni s obje sorte graha. Primjena bakterizacije rezultirala je statistički opravdanim razlikama u broju i masi suhe tvari kvržica, masi nadzemnog dijela biljke i prinosu zrna graha u odnosu na nebakteriziranu kontrolu. Premda se ispitivani autohtoni i referentni sojevi ne razlikuju značajno za većinu svojstava, relativno veće vrijednosti utvrđene su uz primjenu autohtonih sojeva pri čemu se naročito izdvaja soj 1AT. Rezultati istraživanja ukazuju na simbiozni potencijal autohtonih sojeva R. leguminosarum. Potrebna su daljnja istraživanja navedenih sojeva kako bi se izabrali najkvalitetniji za predsjevenu bakterizaciju graha.

Abstract (english)

Although common bean is a very important, high-value vegetable crop, and in Croatia there are favorable agroecological conditions for its cultivation, it is unjustifiably neglected and the market is dominated by imported common bean. The use of symbiotic nitrogen fixation can significantly contribute to environmentally friendly and economically viable common bean production. The aim of this study was to determine the symbiotic properties of different strains of Rhizobium leguminosarum in the agroecological area of Koprivnica-Križevci County. The study was conducted through a field experiment, and the experimental factors were R. leguminosarum strains (four indigenous strains, reference strain and control) and common bean cultivars ('Alida' and 'Marina'). During the experiment, the nodulation ability of strains, dry weight of plant, the number of pods per plant, the number of grains per pod and the grain yield of beans were determined. A statistically justified influence of cultivar on all investigated traits was determined. The cultivar 'Alida' is significantly better than the cultivar 'Marina' in all traits except in the number of grains per pod. The obtained results showed that all investigated strains were compatible with both common bean cultivars. The application of inoculants resulted in statistically justified differences in the number and dry weight of nodules, dry weight of plant and the grain yield of beans in relation to the non-bacterized control. Although the examined indigenous and reference strains do not differ significantly for most traits, relatively higher values were determined with the use of indigenous strains, with strain 1AT being singled out. The results of this study indicate a symbiotic potential of indigenous strains of R. leguminosarum. Further research of these strains is needed to select the best ones for pre-sowing inoculation of common bean.

• Keywords: Phaseolus vulgaris; simbiozna fiksacija dušika; Rhizobium leguminosarum; autohtoni sojevi
• Keywords (english): Phaseolus vulgaris; symbiotic nitrogen fixation; Rhizobium leguminosarum; indigenous strains
• Language: croatian
• URN:NBN: urn:nbn:hr:204:567502
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2022-01-20 08:22:54