Usporedba dvije metode testiranja osjetljivosti repičina sjajnika (Brassicogethes sp.) na insekticide

master's thesis

• Title (english): Comparison of two susceptibility test methods for testing pollen beetle (Brassicogethes sp.) to insecticides
• Author: Mateja Vlaović
• Mentor: Ivan Juran (mentor)
• Committee member: Ivan Juran (predsjednik povjerenstva)
• Committee member: Tanja Gotlin Čuljak (član povjerenstva)
• Committee member: Dinka Grubišić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Agricultural Zoology); Zagreb
• Defense date and country: 2021-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Zbog povećane proizvodnje uljane repice te uzastopne sjetve na istim poljima ili u blizini prošlogodišnjih usjeva došlo je do povećanja populacije repičina sjajnika (Brassicogethes sp.) koji se smatra jednim od najvažnijih štetnika uljane repice. Zbog prečeste primjene insekticida u suzbijanju se javio ozbiljan problem, a to je pojava rezistentnosti repičina sjajnika. Upravo iz tog razloga, cilj ovog istraživanja je utvrditi osjetljivost odraslih oblika repičina sjajnika na aktivne tvari iz skupine piretroida i neonikotinoida različitim test metodama. Populacije odraslih oblika repičinog sjajnika, u svrhu testiranja osjetljivosti na insekticide, prikupljene su na pet lokaliteta u Osječko-baranjskoj županiji. Testiranja su provedena u laboratoriju prema IRAC test metodama 011 za aktivnu tvar lambda-cihalotrin i 021 za acetamiprid. Provedene su dvije test metode od kojih je prva bila metoda tretiranja bočica, a druga metoda umakanja cvatova uljane repice u otopine insekticida. Usporedbom rezultata provedenih metoda utvrđeno je kako metoda tretiranja bočica postiže veću učinkovitost od metode umakanja cvatova. Također, utvrđena je rezistentnost svih testiranih populacija na acetamiprid i osjetljivost dviju populacija na lambda-cihalotrin.

Abstract (english)

Due to the increased production of oilseed rape and successive sowing in the same fields or near last year's crops, there was an increase in the population of pollen beetle (Brassicogethes sp.), which is considered one of the most important pests of oilseed rape. Due to the frequent use of insecticides used for their suppression, a serious problem has arisen, and that is the occurrence of resistance of pollen beetle. For these reasons, the aim of this study is to determine the susceptibility of adult forms of pollen beetle to active substances from the groups of pyrethroids and neonicotinoids by different test methods. Populations of adult forms of pollen beetle were collected at 5 localities in Osijek-Baranja County for the purpose of testing insecticide susceptibility. Testings were conducted in the laboratory according to the IRAC test methods 011 for the active substance lambda-cyhalothrin and 021 for acetamiprid. Two testing methods were used: the first one was the Adult-Vial-Tests, the second one was the method of dipping inflorescences of the oilseed rape into insecticide solutions. By comparing the results of the used methods, it was found that Adult-Vial-Tests achieves greater efficiency than the method of dipping inflorescences. Also, resistance of all tested populations to acetamiprid and susceptibility of the two populations to lambda-cyhalothrin were determined.

• Keywords: uljana repica; rezistentnost; Meligethinae; Test metoda IRAC 011; Test metoda IRAC 021
• Keywords (english): oilseed rape; resistance; Meligethinae; Test method IRAC 011; Test method IRAC 021
• Language: croatian
• URN:NBN: urn:nbn:hr:204:781509
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2021-11-17 11:40:49