Utjecaj abiotskog stresa niskim temperaturama na razinu specijaliziranih metabolita u lišću raštike

master's thesis

• Title (english): Influence of low temperature abiotic stress on the content of specialized metabolites in the leaves of kale
• Author: Marija Zrnić
• Mentor: Nina Toth (mentor)
• Mentor: Dunja Šamec (mentor)
• Committee member: Nina Toth (predsjednik povjerenstva)
• Committee member: Sanja Radman (član povjerenstva)
• Committee member: Jana Šic Žlabur (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Vegetable Crops); Zagreb
• Defense date and country: 2019-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Cilj ovog rada bio je ispitati utjecaj stresa niskim temperaturama na sadržaj specijaliziranih metabolita: glukozinolata, ukupnih fenola, flavonoida, fenolnih kiselina i karotenoida, markera stresa prolina te sadržaja klorofila a i b u lišću raštike (Brassica oleracea var. acephala). Raštika je uzgojena u kontroliranim uvjetima na temperaturi od 21 °C i fotoperiodu od 16 sati dana i 8 sati noći do starosti od 5 i 6 tjedana te podvrgnuta stresu pothlađivanjem (8 °C) i smrzavanjem (-8 °C). Koncentracije metabolita te markera stresa u tkivu listova raštike utvrđene su temeljem UV/VIS spektrometrije. Stres niskim temperaturama utjecao je na sadržaj prolina, ukupnih polifenola, flavonoida, fenolnih kiselina, klorofila a i b te karotenoida no trend i jačina ovise o vrsti stresa te starosti biljke. Općenito, temperaturni stres smrzavanjem djelovao je na smanjenje sadržaja polifenolnih spojeva. Temperatura i starost nisu imale značajan utjecaj na sadržaj glukozinolata. Kod starijih biljaka, stres niskom temperaturom je utjecao na smanjenje sadržaja fotosintetskih pigmenata. Potrebno je provesti daljnja istraživanja koja bi razjasnila utjecaj stresa niskim temperaturama na sadržaj pojedinog metabolita u raštici.

Abstract (english)

The aim of this study was to investigate the effect of low temperature stress on the content of specialized metabolites – glucosinolates, total phenols, flavonoids, phenolic acids and carotenoids, stress markers such as proline and chlorophyll a and b in the leaves of kale (Brassica oleracea var. acephala). Kale was grown in a controlled environment at 21 °C and a photoperiod of 16 hours of light and 8 hours of darkness. 5 and 6 weeks old plants were subjected to chilling (8 °C) and freezing (-8 °C) stress. Metabolite content of kale leaf tissue was determined by UV/VIS spectrometry. Low temperature stress affected the content of proline, total polyphenols, flavonoids, phenolic acids, chlorophyll a and b and carotenoids, but the trend and severity depend on the type of stress and the age of the plant. In general, freezing temperature stress reduced the content of polyphenols. Temperature and age had no significant effect on glucosinolate content. Low temperature stress reduced the content of photosynthetic pigments in older plants. Further research is needed to elucidate the effect of low temperature stress on the content of a particular metabolite in kale.

• Keywords: Brassica oleracea var. acephala; stres niskom temperaturom; prolin; polifenoli; glukozinolati
• Keywords (english): Brassica oleracea var. acephala; low temperature stress; proline; polyphenols; glucosinolates
• Language: croatian
• URN:NBN: urn:nbn:hr:204:880888
• Study programme: Title: Horticulture; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka hortikulture (magistar/ magistra inženjer/inženjerka hortikulture)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-11-01
• Created on: 2019-10-31 11:26:42