Promjena u sastavu karotenoida u listovima bijelih sorata vinove loze

master's thesis

• Title (english): Changes in composition and content of carotenoids during ontogeny in leaves of white varieties of grapevine (Vitis vinifera L.)
• Author: Patricia Baždarić
• Mentor: Jasminka Karoglan Kontić (mentor)
• Committee member: Jasminka Karoglan Kontić (predsjednik povjerenstva)
• Committee member: Marko Karoglan (član povjerenstva)
• Committee member: Željko Andabaka (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Viticulture and Enology); Zagreb
• Defense date and country: 2019-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Karotenoidi su široko rasprostranjeni spojevi koji čine jednu od najvećih grupa pigmenata u prirodi, a u biljkama imaju nezamjenjivu ulogu u procesu fotosinteze. Definiraju se kao C40 izoprenoidi koji svojim djelovanjem doprinose prikupljanju svjetlosne energije sve do reakcijskih središta fotosinteze, apsorbirajući valne duljine od 450 do 500 nm. Najpoznatiji su kao antioksidansi koji štite stanice od fotooksidacijskog oštećenja te imaju sposobnost uklanjanja slobodnih radikala, a time imaju vrlo važnu ulogu u obrambenom sustavu biljke. Prisutnost kartenoida u listovima vinove loze neophodna je za razvoj listova i same biljke te je cilj ovog istraživanja bio utvrditi pojavnost pojedinih karotenoida i promjene u sastavu kod listova vinove loze. Uzorkovanje listova odvijalo se u nekoliko termina, počevši od završetka cvatnje vinove loze, pa sve do razdoblja pred tehnološku zrelost pojedinih sorata vinove loze. Uzorkovano je 10 autohtonih bijelih sorata i dvije introducirane bijele sorte. Listovi su nakon prikupljanja pravilno pohranjeni, liofilizirani te je napravljena ekstrakcija adekvatnom metodom. Ekstrakti su podvrgnuti analizi na tekućinskom kromatografu visoke djelotvornosti. Zadnji korak je uključivao obradu rezultata korištenjem ANOVA testa i post‐hoc Duncanovog testa. Zaključno, utvrđena je značajna razlika između pojedinih sorata u sastavu karotenoida po pojedinim terminima uzorkovanja, kao i značajna razlika kod iste sorte u različitim fazama. Utjecaj okolišnih čimbenika i starenje listova dovodi do pada sadržaja karotenoida te smanjenja fotosintetske aktivnosti.

Abstract (english)

Carotenoids are widely distributed chemical compounds representing one of the largest groups of pigments in nature, playing an irreplaceable role in the photosynthetic activity of plants. They are defined as isoprenoids that contribute to photosynthetic activity by capturing sunlight energy into reaction centers of photosynthesis, absorbing sunlight wavelengths from 450 to 500 nm. Carotenoids are best known as antioxidants which protect the cells from photochemical damage, and they have the ability to remove free radicals and thus play an important role in the plant's defense mechanism. Their presence in grapevine is essential to the development and survival of leaves and plant in general. The aim of this study was to determine the changes in carotenoid compound development in leaves which occurs during ontogeny. Leaf samples were collected periodically, starting from the post‐flowering period and ending just before the technological maturity of grapevine varieties. In this study, samples were taken from 10 autochthonous white varieties and 2 introduced white varieties. Leaf samples were properly stored, lyophilized and later extracted with a proper method. The later analysis was conducted with high‐performance liquid chromatography. The final step included processing the results with ANOVA test in combination with post‐hoc Duncan test. In conclusion, a significant difference was found between the individual. A significant level of difference was also determined in the same grapevine variety observed in different sampling periods. As a result of the environmental conditions influence and the ageing of leaves, carotenoid content is degrading and as well as photosynthetic activity.

• Keywords: vinova loza; bijele sorte; listovi; karotenoidi; tekućinska kromatografija
• Keywords (english): grapevine; white varieties; leaves; carotenoids; liquid chromatography
• Language: croatian
• URN:NBN: urn:nbn:hr:204:145677
• Study programme: Title: Horticulture; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka hortikulture (magistar/ magistra inženjer/inženjerka hortikulture)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-09-23 06:29:45